2-Mercaptoethanol | CAS 60-24-2 | High-Purity Biochemical Solvent

2-Mercaptoethanol, also known as β-Mercaptoethanol or BME, is a key organosulfur compound with the molecular formula C₂H₆OS. It is a clear, hygroscopic liquid widely recognized for its ability to maintain a reducing environment in biochemical applications.

At Aozun Chemical, we provide high-purity 2-Mercaptoethanol (CAS 60-24-2) suitable for molecular biology, protein chemistry, and industrial synthesis.

Key Properties and Specifications

  • CAS Number: 60-24-2

  • Molecular Formula: C₂H₆OS

  • Molecular Weight: 78.13 g/mol

  • Purity: ≥99.0% (GC)

  • Appearance: Clear, colorless to slightly yellow liquid

  • Solubility: Miscible in water and most organic solvents

  • Storage Conditions: Cool, dry, and well-ventilated area; protect from light and air

Primary Applications

1. Protein Electrophoresis (SDS-PAGE)

It is essential in protein analysis. It acts as a potent reducing agent that cleaves disulfide bonds in proteins, allowing for their linearization. This enables accurate molecular weight determination during SDS-PAGE.

2. Enzyme Activity Assays

By preventing the oxidation of free sulfhydryl groups, it helps preserve the native structure and activity of enzymes in various biochemical assays.

3. RNA Extraction and Purification

In molecular biology, 2-Mercaptoethanol is used in lysis buffers to inactivate ribonucleases (RNases) released during cell lysis, thereby protecting intact RNA from degradation.

4. Industrial Intermediate

Beyond the laboratory, it serves as an intermediate in the synthesis of pharmaceuticals, agrochemicals, and polymers.

Quality and Safety

We prioritize quality assurance. Each batch of 2-Mercaptoethanol (CAS 60-24-2) is tested to ensure consistency and high purity.

Handling Guidelines:

  • Hazard Class: Toxic and dangerous for the environment.

  • Personal Protective Equipment (PPE): Safety goggles, chemical-resistant gloves, and adequate ventilation are required during handling.

  • First Aid: In case of contact with skin or eyes, rinse immediately with plenty of water and seek medical attention.

Frequently Asked Questions (FAQs)

1. What is the purpose of 2-Mercaptoethanol in SDS-PAGE?

It is used in SDS-PAGE sample buffers to reduce disulfide bridges within proteins. This breaks the protein’s tertiary and quaternary structures, allowing SDS to bind uniformly and separate proteins based solely on molecular weight.

2. How should it be stored?

It should be stored in a tightly sealed, light-resistant container in a cool, dry area (typically 2–8°C). Because it is hygroscopic and air-sensitive, exposure to air and moisture should be minimized to maintain purity.

3. Is 2-Mercaptoethanol the same as Dithiothreitol (DTT)?

Both are reducing agents used in biochemistry, but they are different. 2-Mercaptoethanol is more volatile and has a stronger odor. DTT is often considered a stronger reducing agent and is effective at lower concentrations. 2-Mercaptoethanol is frequently preferred for electrophoresis due to cost-effectiveness.

4. Can 2-Mercaptoethanol be autoclaved?

No. Due to its volatility and chemical structure, it cannot be autoclaved. Solutions containing 2-Mercaptoethanol should be filter-sterilized if sterile filtration is required.

5. What is the typical shelf life of this product?

When stored unopened under recommended conditions, the shelf life of 2-Mercaptoethanol is typically 24 months from the date of manufacture.

6. Is this product suitable for clinical or human consumption?

No. This product is intended for research and industrial use only. It is not for pharmaceutical, medical, or household use.

Contact Us

For technical data sheets (TDS), safety data sheets (MSDS), or to place an order, please contact our sales team.

Our team at Aozun Chemical is ready to assist with your bulk or research quantity needs for 2-Mercaptoethanol (CAS 60-24-2).